Somatic Embryogenesis and Organogenesis of Biofuel Plant Cassava (Manihot esculenta Crantz) Chinese Cultivars
Rui Mei Li, Rui Duan, Yi Meng Ji, Du Juan Xi, Jiao Liu, Jian Guo, Peng Zhang, Shao Fu
Abstract
Rui Mei Li, Rui Duan, Yi Meng Ji, Du Juan Xi, Jiao Liu, Jian Guo, Peng Zhang, Shao Fu
Abstract
2, 4-D and picloram were compared for their ability to the induction of somatic embryogenesis in Chinese cassava (Manihot esculenta Crantz) cultivars SC5, SC6, SC7 and SC8. In all four cultivars tested, both 2, 4-D and picloram had the capacity to induce primary somatic embryos from axillary buds. And the two hormones were also suitable for subculture of somatic embryos of three cultivars SC5, SC6 and SC8. However both 2, 4-D and picloram can not keep the activity of somatic embryos of cultivar SC7. For organogenesis, cotyledon matured for 10~15 days were better than others.
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2, 4-D and picloram were compared for their ability to the induction of somatic embryogenesis in Chinese cassava (Manihot esculenta Crantz) cultivars SC5, SC6, SC7 and SC8. In all four cultivars tested, both 2, 4-D and picloram had the capacity to induce primary somatic embryos from axillary buds. And the two hormones were also suitable for subculture of somatic embryos of three cultivars SC5, SC6 and SC8. However both 2, 4-D and picloram can not keep the activity of somatic embryos of cultivar SC7. For organogenesis, cotyledon matured for 10~15 days were better than others.
Key concepts: Picloram, Somatic embryogenesis, Cultivar, Cotyledon, Subculture (biology), Organogenesis, Biology, Somatic cell