Effect of Antioxidant of Citri Reticulatae Pericarpium on Cytotoxicity of Oxygen Free Radicals in Cultured NIH3T3 Fibroblast

Objectives : It is demonstrated that oxygen free radicals have cytotoxic effect on NIH3T3 fibroblast cells. Recently, many of herb extracts have an effect of antioxidant in oxygen free radical-induced cytotoxicity. But, the toxic mechanism of oxygen free radical is left unknown. The purpose of this study was to examine the cytotoxicity of hydrogen peroxide () and antioxidant effect of Citri reticulatae pericarpium (CRP) on NIH3T3 fibroblasts. Methods : The cytotoxicy was measured by cell viability by XTT assay in NIH3T3 fibroblasts. XTT assay is regarded as a very sensitive screening method for the determination of the cell viability on various chemicals. Results : In this study, H2O2 decreased cell viability according to the dose- and time dependent manners after NIH3T3 fibroblasts were treated with various concentrations of H2O2 for 4 hours. And also, CRP showed the effect of antioxidant on cytotoxicity in cultured NIH3T3 fibroblasts. Conclusion : These results suggest that has highly cytotoxic effect on cultured NIH3T3 fibroblasts by the decrease of cell viavility, and the herb extract such as CRP was showed the effect of antioxidant on cytotoxicity in these cultures.