Effect of Kaempferol on the Cytotoxicity Induced Oxygen Free Radicals in Skin Fibroblast Derived from Human In Vitro

In order to evaluate on the effect of kaempferol on the cytotoxicity of oxygen free radicals, XTT assay was performed to determine the cell viability after skin fibroblasts derived from human (Detroit 51) that were treated with various concentrations of hydrogen peroxide (H₂O₂). And also, the effect of kaempferol on the cytotoxicity induced by H₂O₂ that was examined by cell viability, lactate dehydrogenase (LDH) activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity in these cultures. H₂O₂ decreased cell viability in dose-dependent manner in these cultures and the XTT?? and XTT?? values were determined at concentration of 35 μM and 90 μM of H₂O₂ after skin fibroblasts derived from human were treated with 15~90 μM of H₂O₂ for 6 hours, respectively. H₂O₂ was highly toxic on cultured skin fibroblasts derived from human by toxic criteria of Brenfreund and Puerner (1984). In the protective effect of kaempferol on H₂O₂-induced cytotoxicity, kaempferol increased DPPH radical scavenging activity and significantly decreased LDH activity. From these results, it is suggested that oxygen free radical, H₂O₂, was highly toxic on cultured skin fibroblasts derived from human, and also kaempferol of flavonoid showed the protection on H₂O₂-induced cytotoxicity.