MICELLE-MEDIATED EXTRACTION FOR THE ANALYSIS OF CHLOROGENIC ACID, RUTIN, AND QUERCETIN IN HONEYSUCKLE BY HPLC–UV

A MME-HPLC (UV) method was developed for the determination of chlorogenic acid, rutin, and quercetin; nonionic surfactant Triton X-100 was used to extract and preconcentrate active ingredients from Honeysuckle prior to their determination by HPLC-UV. The experimental conditions were optimized by a L16 (45) orthogonal array design (OAD) with five factors such as concentration and kind of TX, liquid/solid ratio, extraction temperature, and time at four levels using statistical analysis. Under optimum conditions, that is, 5% Triton X-100 (w/v), liquid/solid ratio of 400:1 (mL · g−1), ultrasonic-assisted extraction for 30 min at 30°C, the extraction content of the active ingredients reached the highest value. When compared with commonly used solvents, 5% Triton X-100 yielded, in a way, higher than the extraction efficiency of methanol. For the preconcentration of compounds by cloud-point extraction, sodium chloride was added to the solution to facilitate the phase separation. The preconcentration factor for each compound was about 12.5. The proposed method has been applied to the determination of active ingredients in different samples and a standard reference material (SRM).