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A New Embryo Rescue Procedure for Interspecific Hybridization

Regine Mathias, Sonsoles Espinosa, G. Röbbelen

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Abstract

Abstract A new technique is presented that allows to overcome very early embryo abortion after interspecific crosses. Embryogenic sectors of young fertilized ovules are cultivated in vitro on a solid/liquid double layer culture medium containing activated charcoal in the solid phase. Hybrid plans are obtained afer transfer the developed for the interspecific cross Cuphea paucipetala greenwooodii×C. laminuligera; but it is most likely also applicable to other combinations, even in different genera.

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What this paper is about

Abstract A new technique is presented that allows to overcome very early embryo abortion after interspecific crosses. Embryogenic sectors of young fertilized ovules are cultivated in vitro on a solid/liquid double layer culture medium containing activated charcoal in the solid phase. Hybrid plans are obtained afer transfer the developed for the interspecific cross Cuphea paucipetala greenwooodii×C. laminuligera; but it is most likely also applicable to other combinations, even in different genera.

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Available abstract

Abstract A new technique is presented that allows to overcome very early embryo abortion after interspecific crosses. Embryogenic sectors of young fertilized ovules are cultivated in vitro on a solid/liquid double layer culture medium containing activated charcoal in the solid phase. Hybrid plans are obtained afer transfer the developed for the interspecific cross Cuphea paucipetala greenwooodii×C. laminuligera; but it is most likely also applicable to other combinations, even in different genera.

Key concepts: Embryo rescue, Biology, Interspecific competition, Interspecific hybridization, Ovule, Embryo, Botany, Gracilaria

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