Research on the Technique of the Embryo Rescue of Seedless Grape

[Objective]The reference for the future conduct of the seedless grape breeding with the method of embryo rescue was provided through the study on the main factor in the process of embryo rescue.[Method]The immature ovule of six seedless grape hybrids and inbred combinations being used as experimental material,the effect of the culturing time of ovule,medium and hormone concentration on its embryo rescue was studied.[Results]The appropriate culturing time of the ovule of these materials was the 40th day after flowering in Huoxing-Wuhe × Wuhe-Baijixing,the 55th day after flowering in Jinxing-Wuhe ×Kangneng-Wuhe and the 45th day after flowering in Youwuhe × Brown-Wuhe.The medium-ER as the basic medium was more conducive to ovule development and germination.The demand of different varieties to the concentration of IBA was different.The lower concentration of IBA was required in well-developed embryo and the later abortive ovule and higher concentration of IBA was suitable for the less developed embryo and early abortive ovule.[Conclusion]The culturing time of ovule,the type of medium and the culturing condition was very important in the success of grape embryo rescue and the establishment of the condition for its success depended on the specific varieties.