1997•Journal of Liquid Chromatography & Related TechnologiesRequires access

Separation of D and L Enantiomers of [(Phenylsulfonyl)amino]-3-oxo-1-octadecanol by Chiral High Performance Liquid Chromatography

Fred G. Rojas, Russell C. Klix, A. V. BHATIA

Abstract

A convenient and rapid method has been developed for the separation of the D and L enantiomers of [(phenyl-sulfonyl)amino]-3-oxo-octadecanol using chiral High Performance Liquid Chromatography (HPLC). An amylose tris(3,5-dimethylphenylcarbamate) chiral HPLC column was used for the separation. The two enantiomers were fully resolved from each other with approximate retention times of 11 and 13 minutes for the L and the D forms respectively. The total run time per sample was 30 minutes.

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Key concepts: Chemistry, Enantiomer, Chromatography, High-performance liquid chromatography, Chiral column chromatography, Chiral derivatizing agent, Sulfonyl, Chiral stationary phase