2013•Advanced materials researchOpen access

Research of Producing Single Cell Protein with Molasses Wastewater

Yi Yao, Hua Lin, Liang Tan, Shao Hong You, Liao Zhang

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Abstract

This paper is to study the optimum conditions for extracting the protein feed by using the mixed microbial fermentation. And using the single factor and orthogonal methods, the microbial content as the main indicators, investigated the effects of the combination of strain, strain ratio, the amount of bacteria, fermentation temperature, the concentration of fermentation, fermentation pH, nitrogen sources and inorganic salts and other factors on the fermentation of protein yield. The test results showed that the best fermentation process is sterilization process, the best combination of strains of Geotrichum candidum, Candida tropicalis and Candida utilis yeast, the cast of bacteria was 10%, the best molasses fermentation concentration 20g / 100 mL, and temperature 30 ° C, initial pH 5.0, appropriate amount of Yeast extract, magnesium sulfate, potassium dihydrogen phosphate and super phosphate. The Test provides a theoretical and experimental basis for Molasses wastewater utilization

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What this paper is about

This paper is to study the optimum conditions for extracting the protein feed by using the mixed microbial fermentation. And using the single factor and orthogonal methods, the microbial content as the main indicators, investigated the effects of the combination of strain, strain ratio, the amount of bacteria, fermentation temperature, the concentration of fermentation, fermentation pH, nitrogen sources and inorganic salts and other factors on the fermentation of protein yield. The test results showed that the best fermentation process is sterilization process, the best combination of strains of Geotrichum candidum, Candida tropicalis and Candida utilis yeast, the cast of bacteria was 10%, the best molasses fermentation concentration 20g / 100 mL, and temperature 30 ° C, initial pH 5.0, appropriate amount of Yeast extract, magnesium sulfate, potassium dihydrogen phosphate and super phosphate. The Test provides a theoretical and experimental basis for Molasses wastewater utilization

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Available abstract

This paper is to study the optimum conditions for extracting the protein feed by using the mixed microbial fermentation. And using the single factor and orthogonal methods, the microbial content as the main indicators, investigated the effects of the combination of strain, strain ratio, the amount of bacteria, fermentation temperature, the concentration of fermentation, fermentation pH, nitrogen sources and inorganic salts and other factors on the fermentation of protein yield. The test results showed that the best fermentation process is sterilization process, the best combination of strains of Geotrichum candidum, Candida tropicalis and Candida utilis yeast, the cast of bacteria was 10%, the best molasses fermentation concentration 20g / 100 mL, and temperature 30 ° C, initial pH 5.0, appropriate amount of Yeast extract, magnesium sulfate, potassium dihydrogen phosphate and super phosphate. The Test provides a theoretical and experimental basis for Molasses wastewater utilization

Key concepts: Fermentation, Geotrichum, Candida tropicalis, Bacteria, Yeast, Food science, Chemistry, Single-cell protein

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