2006•Japanese Journal of Applied PhysicsOpen access

Observation of Hybridization on a DNA Array by Surface Plasmon Resonace Imaging using Au Nanoparticles

Fumio Nakamura, Masateru Ito, Ablihito Manna, Kaoru Tamada, Masahiko Hara, Wolfgang Knoll

Abstract

To realize the sensitive detection of DNA hybridization, DNA-modified Au nanoparticles (DNA-AuNPs) containing single- and double-stranded portions were prepared. The hybridization of a target DNA using the DNA-AuNPs on a DNA self-assembled monolayer (DNA-SAM) was monitored in situ by surface plasmon resonance (SPR) imaging. A sandwich type hybridization (DNA-SAM/target DNA/DNA-AuNP) experiment was carried out in order to enhance the hybridization signal. SPR imaging results strongly indicate that the hybridization signal is enhanced several times compared to the case of target DNA hybridization.

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Available abstract

Key concepts: DNA–DNA hybridization, DNA, Surface plasmon resonance, In situ hybridization, Nucleic acid thermodynamics, Hybridization probe, Monolayer, Nanoparticle