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CHICKEN INTERLEUKIN-1 BETA PRODUCING CELLS DETECLED BY NUCLEIC ACID SPOT HYBRIDIZATION AND HYBRIDIZATION IN SITU

Xu Xiao-Jing

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Abstract

Complementary DNA were designed according to the described interleukin-1β sequence of mammals,such as human,murine,equine,bovine,ovine,rabbit. 32 P Labelled DNA probes were made,and were used to detect the IL-1β producing cells of chick brain tissues infected with Avian Encephalomyelitis Virus (AEV)by means of nucleic acid spot hybridization and hybridization in situ.The results indicated that hybridization reactions were positive.

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What this paper is about

Complementary DNA were designed according to the described interleukin-1β sequence of mammals,such as human,murine,equine,bovine,ovine,rabbit. 32 P Labelled DNA probes were made,and were used to detect the IL-1β producing cells of chick brain tissues infected with Avian Encephalomyelitis Virus (AEV)by means of nucleic acid spot hybridization and hybridization in situ.The results indicated that hybridization reactions were positive.

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Available abstract

Complementary DNA were designed according to the described interleukin-1β sequence of mammals,such as human,murine,equine,bovine,ovine,rabbit. 32 P Labelled DNA probes were made,and were used to detect the IL-1β producing cells of chick brain tissues infected with Avian Encephalomyelitis Virus (AEV)by means of nucleic acid spot hybridization and hybridization in situ.The results indicated that hybridization reactions were positive.

Key concepts: In situ hybridization, Nucleic acid, Nucleic acid thermodynamics, Molecular biology, Biology, DNA–DNA hybridization, DNA, Hybridization probe

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CHICKEN INTERLEUKIN-1 BETA PRODUCING CELLS DETECLED BY NUCLEIC ACID SPOT HYBRIDIZATION AND HYBRIDIZATION IN SITU — Research Paper | ScholarLens