1999•Journal of Clinical MicrobiologyOpen access

Rapid Identification of Campylobacter , Arcobacter , and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene

Stephen M. Marshall, Pasquale L. Melito, David L. Woodward, Wendy M. Johnson, Frank G. Rodgers, Michael R. Mulvey

Abstract

A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter, and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified by this assay.

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Key concepts: Campylobacter, Restriction fragment length polymorphism, Biology, Arcobacter, Terminal restriction fragment length polymorphism, 16S ribosomal RNA, Helicobacter, Polymerase chain reaction