2012•Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca AgricultureOpen access

Trichoderma Reesei Cellulase Produced by Submerged Versus Solid State Fermentations

Diana NEAGU, Jacqueline Destain, Phillipe Thonart, Carmen Socaciu

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Abstract

The aim of this study was to produce and characterize a cellulase-rich fraction using submerged or solid state fermentation of Trichoderma reesei (QM 1914) strain. The carbon sources were the wheat bran or sawdust, the production yield of this enzyme production was higher in both fermentation types using sawdust substrate, and especially by solid state fermentation, after five days of fermentation. The optimum pH and temperature for the efficient crude enzyme production was established to be 5 and 60°C, respectively, but lost 50% of its activity after 30 minutes, when heated at 60°C. Comparatively with other fungi, the efficiency of Trichoderma sp. to synthesize cellulase rich extract was higher.

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What this paper is about

The aim of this study was to produce and characterize a cellulase-rich fraction using submerged or solid state fermentation of Trichoderma reesei (QM 1914) strain. The carbon sources were the wheat bran or sawdust, the production yield of this enzyme production was higher in both fermentation types using sawdust substrate, and especially by solid state fermentation, after five days of fermentation. The optimum pH and temperature for the efficient crude enzyme production was established to be 5 and 60°C, respectively, but lost 50% of its activity after 30 minutes, when heated at 60°C. Comparatively with other fungi, the efficiency of Trichoderma sp. to synthesize cellulase rich extract was higher.

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Available abstract

The aim of this study was to produce and characterize a cellulase-rich fraction using submerged or solid state fermentation of Trichoderma reesei (QM 1914) strain. The carbon sources were the wheat bran or sawdust, the production yield of this enzyme production was higher in both fermentation types using sawdust substrate, and especially by solid state fermentation, after five days of fermentation. The optimum pH and temperature for the efficient crude enzyme production was established to be 5 and 60°C, respectively, but lost 50% of its activity after 30 minutes, when heated at 60°C. Comparatively with other fungi, the efficiency of Trichoderma sp. to synthesize cellulase rich extract was higher.

Key concepts: Trichoderma reesei, Cellulase, Solid-state fermentation, Bran, Sawdust, Fermentation, Trichoderma, Food science

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