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Comparative evaluation of PCR and ELISA for diagnosing bovine brucellosis.

Shubhada K. Chothe, Hari Mohan Saxena, Deepti Chachra

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Abstract

200 Serum samples from animals with Brucella infection to ascertain the effectiveness and suitability of Polymerase Chain Reaction (PCR) and commercially available Enzyme linked Immunosorbent Assay (ELISA) kits. In PCR, only 3 out the 200 samples showed a band of size 905bp, typical of Brucella species. Other samples failed to show positive reaction by PCR. In comparison, ELISA could detect 75 out of the 200 samples as positive. The results indicate that ELISA is better compared to PCR for screening animals for Brucellosis.

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What this paper is about

200 Serum samples from animals with Brucella infection to ascertain the effectiveness and suitability of Polymerase Chain Reaction (PCR) and commercially available Enzyme linked Immunosorbent Assay (ELISA) kits. In PCR, only 3 out the 200 samples showed a band of size 905bp, typical of Brucella species. Other samples failed to show positive reaction by PCR. In comparison, ELISA could detect 75 out of the 200 samples as positive. The results indicate that ELISA is better compared to PCR for screening animals for Brucellosis.

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Available abstract

200 Serum samples from animals with Brucella infection to ascertain the effectiveness and suitability of Polymerase Chain Reaction (PCR) and commercially available Enzyme linked Immunosorbent Assay (ELISA) kits. In PCR, only 3 out the 200 samples showed a band of size 905bp, typical of Brucella species. Other samples failed to show positive reaction by PCR. In comparison, ELISA could detect 75 out of the 200 samples as positive. The results indicate that ELISA is better compared to PCR for screening animals for Brucellosis.

Key concepts: Brucellosis, Brucella, Polymerase chain reaction, Brucella abortus, Biology, Virology, Brucellaceae, Microbiology

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