ScholarLens Back to search
2008•Journal of Immunology and ImmunopathologyRequires access

Evaluation of the comparative efficacy of recombinant protein antigens based enzyme-linked immunosorbent assay for the diagnosis of caprine leptospirosis

Surya Sankar, Hiron M. Harshan, E Thankapandian, Pallab Chaudhary, Satish K. Srivastava

Open publisher page 0 citations

Abstract

A comparative study was done between LipL32 and LipL41 recombinant antigens based enzyme-linked immunosorbent assay (ELISA) with respect to the standard reference test-Microscopic Agglutination Test (MAT), using caprine serum samples. MAT was conducted using 11 Leptospira serovars. The LipL32 and LipL41 genes were amplified using specific primers and the polymerase chain reaction products (PCR) were then cloned and expressed. The optimum antigen concentration of 100 ng of each antigen was used for the screening of serum samples from goat suspected of leptospirosis. It was found that LipL32 recombinant antigen based ELISA detected more positive cases of illness than MAT.

About this research paper

What this paper is about

A comparative study was done between LipL32 and LipL41 recombinant antigens based enzyme-linked immunosorbent assay (ELISA) with respect to the standard reference test-Microscopic Agglutination Test (MAT), using caprine serum samples. MAT was conducted using 11 Leptospira serovars. The LipL32 and LipL41 genes were amplified using specific primers and the polymerase chain reaction products (PCR) were then cloned and expressed. The optimum antigen concentration of 100 ng of each antigen was used for the screening of serum samples from goat suspected of leptospirosis. It was found that LipL32 recombinant antigen based ELISA detected more positive cases of illness than MAT.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A comparative study was done between LipL32 and LipL41 recombinant antigens based enzyme-linked immunosorbent assay (ELISA) with respect to the standard reference test-Microscopic Agglutination Test (MAT), using caprine serum samples. MAT was conducted using 11 Leptospira serovars. The LipL32 and LipL41 genes were amplified using specific primers and the polymerase chain reaction products (PCR) were then cloned and expressed. The optimum antigen concentration of 100 ng of each antigen was used for the screening of serum samples from goat suspected of leptospirosis. It was found that LipL32 recombinant antigen based ELISA detected more positive cases of illness than MAT.

Key concepts: Direct agglutination test, Recombinant DNA, Antigen, Serotype, Leptospira, Polymerase chain reaction, Leptospirosis, Virology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Evaluation of the comparative efficacy of recombinant protein antigens based enzyme-linked immunosorbent assay for the diagnosis of caprine leptospirosis — Research Paper | ScholarLens