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Cold Staining And Ziehl - Neelsen Staining For Acid Fast Bacilli

Rajvir Singh, Santosh K. Katiyar, Sudhir Chaudhri, Sharma Alka, Denish Sharma, Mital Richa

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Abstract

The cold staining was compared with Ziehl-Neelsen (Z-N) method for detection of acid fast bacilli (AFB) in sputum in 1500 sputum smears. In cold staining method 72.1% cases were reported positive for AFB and 27.9% negative, while in Z-N method 75% were reported positive and 25% negative and the difference was not statistically significant (P>0.05).

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What this paper is about

The cold staining was compared with Ziehl-Neelsen (Z-N) method for detection of acid fast bacilli (AFB) in sputum in 1500 sputum smears. In cold staining method 72.1% cases were reported positive for AFB and 27.9% negative, while in Z-N method 75% were reported positive and 25% negative and the difference was not statistically significant (P>0.05).

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Available abstract

The cold staining was compared with Ziehl-Neelsen (Z-N) method for detection of acid fast bacilli (AFB) in sputum in 1500 sputum smears. In cold staining method 72.1% cases were reported positive for AFB and 27.9% negative, while in Z-N method 75% were reported positive and 25% negative and the difference was not statistically significant (P>0.05).

Key concepts: Staining, Ziehl–Neelsen stain, Acid-fast, Sputum, Medicine, Bacilli, Pathology, Microbiology

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