Cold Staining And Ziehl - Neelsen Staining For Acid Fast Bacilli
Rajvir Singh, Santosh K. Katiyar, Sudhir Chaudhri, Sharma Alka, Denish Sharma, Mital Richa
Abstract
Rajvir Singh, Santosh K. Katiyar, Sudhir Chaudhri, Sharma Alka, Denish Sharma, Mital Richa
Abstract
The cold staining was compared with Ziehl-Neelsen (Z-N) method for detection of acid fast bacilli (AFB) in sputum in 1500 sputum smears. In cold staining method 72.1% cases were reported positive for AFB and 27.9% negative, while in Z-N method 75% were reported positive and 25% negative and the difference was not statistically significant (P>0.05).
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The cold staining was compared with Ziehl-Neelsen (Z-N) method for detection of acid fast bacilli (AFB) in sputum in 1500 sputum smears. In cold staining method 72.1% cases were reported positive for AFB and 27.9% negative, while in Z-N method 75% were reported positive and 25% negative and the difference was not statistically significant (P>0.05).
Key concepts: Staining, Ziehl–Neelsen stain, Acid-fast, Sputum, Medicine, Bacilli, Pathology, Microbiology