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CALLUS INDUCTION AND BROWNING INHIBITION IN ILEX KUDINGCHA C. J. TSENG

Zhu Ji, Yao Ruhua, Yuwen Huang, Liang Chengye

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Abstract

Young leaf segments from miropropagated plants were used to study the optimum medium and growth regulators for callus induction of Ilex kudingcha. The highest multiplication rate for callus induction and subculture of calluses was obtained using MS medium supplemented with 2.0 mg L~(-1) BA and 4.0 mg L~(-1) NAA. 0.1% phytic acid could markedly promote the growth of calluses and inhibit callus browning. Multiplication rate of calluses was highest in calluses cultured for 40-50 days, and decreased after 27~(th) generation in subculture.

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What this paper is about

Young leaf segments from miropropagated plants were used to study the optimum medium and growth regulators for callus induction of Ilex kudingcha. The highest multiplication rate for callus induction and subculture of calluses was obtained using MS medium supplemented with 2.0 mg L~(-1) BA and 4.0 mg L~(-1) NAA. 0.1% phytic acid could markedly promote the growth of calluses and inhibit callus browning. Multiplication rate of calluses was highest in calluses cultured for 40-50 days, and decreased after 27~(th) generation in subculture.

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Available abstract

Young leaf segments from miropropagated plants were used to study the optimum medium and growth regulators for callus induction of Ilex kudingcha. The highest multiplication rate for callus induction and subculture of calluses was obtained using MS medium supplemented with 2.0 mg L~(-1) BA and 4.0 mg L~(-1) NAA. 0.1% phytic acid could markedly promote the growth of calluses and inhibit callus browning. Multiplication rate of calluses was highest in calluses cultured for 40-50 days, and decreased after 27~(th) generation in subculture.

Key concepts: Callus, Subculture (biology), Browning, Botany, Tissue culture, Chemistry, Biology, Horticulture

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