Solid phase extraction using a column with a monolithic molecuraly imprinted epoxy resin-based polymeric stationary phase : HPLC determination of malachite green in fish and water samples

A column with a novel monolithic molecularly imprinted polymer (MIP)-based stationary phase with malachite green (MG) as template molecules was prepared. The synthesized MIP was characterized by IR and SEM. Extraction conditions on MIPs were optimized. MG and some other compounds were employed for selectivity tests. River water samples were spiked with MG at the concentration 20-500 ng mL -1 ; extraction was performed using a MISPE column and detection was accomplished by HPLC. The achieved recoveries were in the range 95.06-100.13%; relative standard deviation was 0.025-6.63%. MG concentrations in three natural water and fish samples were 2.65, 2.60, 2.36 and 5.63 ng mL -1 , respectively.