ScholarLens Back to search
1964•Journal of BacteriologyOpen access

TRANSFORMATION TO PROTOTROPHY AND POLYGLUTAMIC ACID SYNTHESIS IN BACILLUS LICHENIFORMIS

Carmen Gomez Leonard, D. K. Mattheis, M. J. Mattheis, Riley D. Housewright

Open full text 20 citations

Abstract

Leonard, C. Gomez (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), D. K. Mattheis, M. J. Mattheis, and R. D. Housewright. Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. J. Bacteriol. 88:220-225. 1964.-Growth conditions necessary for transformation to prototrophy of 14 auxotrophs of Bacillus licheniformis were studied. The unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is discussed. Under optimal growth conditions for a serine-deficient mutant transformation frequencies of 0.1% were obtained. A medium for transformation of competent cells in liquid suspensions is described. In addition, this report presents evidence for the transformation of three nonencapsulated mutants of B. licheniformis for the ability to synthesize polyglutamic acid (capsular material).

About this research paper

What this paper is about

Leonard, C. Gomez (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), D. K. Mattheis, M. J. Mattheis, and R. D. Housewright. Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. J. Bacteriol. 88:220-225. 1964.-Growth conditions necessary for transformation to prototrophy of 14 auxotrophs of Bacillus licheniformis were studied. The unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is discussed. Under optimal growth conditions for a serine-deficient mutant transformation frequencies of 0.1% were obtained. A medium for transformation of competent cells in liquid suspensions is described. In addition, this report presents evidence for the transformation of three nonencapsulated mutants of B. licheniformis for the ability to synthesize polyglutamic acid (capsular material).

Why it matters

OpenAlex reports 20 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Leonard, C. Gomez (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), D. K. Mattheis, M. J. Mattheis, and R. D. Housewright. Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. J. Bacteriol. 88:220-225. 1964.-Growth conditions necessary for transformation to prototrophy of 14 auxotrophs of Bacillus licheniformis were studied. The unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is discussed. Under optimal growth conditions for a serine-deficient mutant transformation frequencies of 0.1% were obtained. A medium for transformation of competent cells in liquid suspensions is described. In addition, this report presents evidence for the transformation of three nonencapsulated mutants of B. licheniformis for the ability to synthesize polyglutamic acid (capsular material).

Key concepts: Auxotrophy, Bacillus licheniformis, Polyglutamic acid, Transformation (genetics), Biology, Mutant, Biochemistry, Microbiology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
TRANSFORMATION TO PROTOTROPHY AND POLYGLUTAMIC ACID SYNTHESIS IN BACILLUS LICHENIFORMIS — Research Paper | ScholarLens