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SCREENING OF BLACKGRAM GENOTYPES UNDER ARTIFICIALLY INOCULATED CONDITIONS AND BASED ON MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE

L. Prasanthi, B.V. Bhaskara Reddy, Rekha Rani Kokkanti, T. Rajeswari, Y. Sivaprasad, K. Raja Reddy

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Abstract

Powdery mildew is an important disease in blackgram which causes severe yield loses. Screening of 15 lines along with LBG 17 resistant check for powdery mildew under artificial inoculation identified 3 lines having 0–2% plant disease incidence and grouped under 0–1 disease score. PCR reactions using different primers with genomic DNA of these lines resulted in identification of three resistant sources with specific amplification for resistance to powdery mildew at 900bp with OPT16 primer. Considering the powdery mildew reaction and resistance linked RAPD marker, it was possible to identify the new resistance sources in a short time and they could be utilized in breeding programme or for direct release.

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What this paper is about

Powdery mildew is an important disease in blackgram which causes severe yield loses. Screening of 15 lines along with LBG 17 resistant check for powdery mildew under artificial inoculation identified 3 lines having 0–2% plant disease incidence and grouped under 0–1 disease score. PCR reactions using different primers with genomic DNA of these lines resulted in identification of three resistant sources with specific amplification for resistance to powdery mildew at 900bp with OPT16 primer. Considering the powdery mildew reaction and resistance linked RAPD marker, it was possible to identify the new resistance sources in a short time and they could be utilized in breeding programme or for direct release.

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Available abstract

Powdery mildew is an important disease in blackgram which causes severe yield loses. Screening of 15 lines along with LBG 17 resistant check for powdery mildew under artificial inoculation identified 3 lines having 0–2% plant disease incidence and grouped under 0–1 disease score. PCR reactions using different primers with genomic DNA of these lines resulted in identification of three resistant sources with specific amplification for resistance to powdery mildew at 900bp with OPT16 primer. Considering the powdery mildew reaction and resistance linked RAPD marker, it was possible to identify the new resistance sources in a short time and they could be utilized in breeding programme or for direct release.

Key concepts: Powdery mildew, Inoculation, Biology, RAPD, Plant disease resistance, Primer (cosmetics), Genotype, Molecular marker

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SCREENING OF BLACKGRAM GENOTYPES UNDER ARTIFICIALLY INOCULATED CONDITIONS AND BASED ON MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE — Research Paper | ScholarLens