2015•Scholar Science Journals - International Journal of Biomedical ResearchOpen access

Comparitive study of UV-Visible spectrophotometry and high performance liquid Chromatography methods for quantitative estimation of paracetamol in a tablet formulation

Chelamalla Radhika, Akena Venkatesham

Abstract

The objective of present study was to analyse the comparision between high performance liquid chromatography (HPLC) and UV-Spectrophotometry for quantitative determination of paracetamol in a marketed formulation.Rp-HPLC method involved a reversed-phase XBD C18 column thermostated at 25 °C, UV detection at 230 nm, flow rate of 1.0 ml/min and a mobile phase acetonitrile-water (25:75) was used.These methods showed good linearity over the concentration range of 2-20 μg/mL.The linearity was obtained for paracetamol by reverse phase high performance liquid chromatography (RP-HPLC) R 2 =0.993 and by UV -Spectrophotometry R 2 =0.977.The precision and recoveries of paracetamol for HPLC and UV-Spectrophotometry methods were in the range of 99.00 -100.10 % and 99.71-100.95%.Among these two methods high performance liquid chromatography method of analysis showed reliable results for quantitative etermination of paracetamol in a tablet formulation.

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Key concepts: Chromatography, High-performance liquid chromatography, Spectrophotometry, Chemistry, Quantitative analysis (chemistry), Acetonitrile