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Cloning and Mapping of EcoRI, HindIII, and PstI Fragments of Bovine Herpesvirus 4 (DN-599) Genome

Vicky L. van Santen, Li-Yun Chang

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Abstract

EcoRI, HindIII, and PstI restriction fragments representing the entire genome of the DN-599 isolate, the American prototype strain, of bovine herpesvirus 4 (BHV-4) were cloned. These cloned restriction fragments were analyzed by restriction enzyme digestion, hybridization to Southern blots of BHV-4 (DN-599) DNA, and cross-hybridization of cloned fragments. The resulting data were used to construct restriction maps of the unique region of BHV-4 (DN-599) DNA for restriction enzymes EcoRI, HindIII, and PstI. The EcoRI and HindIII restriction maps confirm those previously deduced for the DN-599 isolate by hybridization of cloned fragments of a European strain of BHV-4 to Southern blots of DN-599 DNA. The PstI restriction map is the first reported for BHV-4.

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What this paper is about

EcoRI, HindIII, and PstI restriction fragments representing the entire genome of the DN-599 isolate, the American prototype strain, of bovine herpesvirus 4 (BHV-4) were cloned. These cloned restriction fragments were analyzed by restriction enzyme digestion, hybridization to Southern blots of BHV-4 (DN-599) DNA, and cross-hybridization of cloned fragments. The resulting data were used to construct restriction maps of the unique region of BHV-4 (DN-599) DNA for restriction enzymes EcoRI, HindIII, and PstI. The EcoRI and HindIII restriction maps confirm those previously deduced for the DN-599 isolate by hybridization of cloned fragments of a European strain of BHV-4 to Southern blots of DN-599 DNA. The PstI restriction map is the first reported for BHV-4.

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Available abstract

EcoRI, HindIII, and PstI restriction fragments representing the entire genome of the DN-599 isolate, the American prototype strain, of bovine herpesvirus 4 (BHV-4) were cloned. These cloned restriction fragments were analyzed by restriction enzyme digestion, hybridization to Southern blots of BHV-4 (DN-599) DNA, and cross-hybridization of cloned fragments. The resulting data were used to construct restriction maps of the unique region of BHV-4 (DN-599) DNA for restriction enzymes EcoRI, HindIII, and PstI. The EcoRI and HindIII restriction maps confirm those previously deduced for the DN-599 isolate by hybridization of cloned fragments of a European strain of BHV-4 to Southern blots of DN-599 DNA. The PstI restriction map is the first reported for BHV-4.

Key concepts: EcoRI, PstI, HindIII, Restriction enzyme, Molecular biology, Southern blot, Restriction map, Restriction site

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Cloning and Mapping of EcoRI, HindIII, and PstI Fragments of Bovine Herpesvirus 4 (DN-599) Genome — Research Paper | ScholarLens