2005•华中科技大学学报：医学英德文版Requires access
Biological Characteristics of Human Bone Marrow Mesenchymal Stem Cell Cultured in Vitro
法宪恩, 王利霞, 侯剑峰, 张瑞成, 王海永, 杨辰垣
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Abstract
Summary: Some biological characteristics of human bone marrow mesenchymal stem cells (MSCs) cultured in vitro were observed. hMSCs were isolated from bone marrow and purified by density gradient centrifugation method, and then cultured in vitro. The proliferation and growth characteristics of hMSCs were observed in primary and passage culture. MSCs of passage 3 were examined for the purify by positive rate of CD29 and CD44 through flow cytometry. Human bone marrow MSCs showed active proliferation capacity in vitro. The purify of MSCs separated by our method was higher than 90 %. It was concluded that hMSCs have been successfully cultured and expanded effectively. It provided a foundation for further investigation and application of MSCs.