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PRESERVATION AND STORAGE OF BLOOD SAMPLES CONTAINING ALCOHOL

H M Stone, J M Muirhead, Hilary Thompson

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Abstract

Although sodium fluoride protects blood samples from changes in alcohol content arising from microbial action, small losses of alcohol due to oxidation occur in stored blood samples. In controlled laboratory experiments oxidative losses were minimised by the incorporation of sodium azide into the blood sample at a concentration between 0.15 and 0.3%. In blood alcohol standards (100.6 mg alcohol per 100 ml blood) prepared with sodium azide, and stored at 4 deg C for up to 3 months, alcohol losses amounted to only 1.65 plus or minus 0.15 mg/100 ml.

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What this paper is about

Although sodium fluoride protects blood samples from changes in alcohol content arising from microbial action, small losses of alcohol due to oxidation occur in stored blood samples. In controlled laboratory experiments oxidative losses were minimised by the incorporation of sodium azide into the blood sample at a concentration between 0.15 and 0.3%. In blood alcohol standards (100.6 mg alcohol per 100 ml blood) prepared with sodium azide, and stored at 4 deg C for up to 3 months, alcohol losses amounted to only 1.65 plus or minus 0.15 mg/100 ml.

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Available abstract

Although sodium fluoride protects blood samples from changes in alcohol content arising from microbial action, small losses of alcohol due to oxidation occur in stored blood samples. In controlled laboratory experiments oxidative losses were minimised by the incorporation of sodium azide into the blood sample at a concentration between 0.15 and 0.3%. In blood alcohol standards (100.6 mg alcohol per 100 ml blood) prepared with sodium azide, and stored at 4 deg C for up to 3 months, alcohol losses amounted to only 1.65 plus or minus 0.15 mg/100 ml.

Key concepts: Sodium azide, Alcohol, Chemistry, Sodium fluoride, Sodium, Ethanol, Blood alcohol, Azide

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