Callus induction from Ipomoea aquatica Forsk. leaf and its antioxidant activity

Callus from the leaves of Ipomoea aquatica Forsk. was initiated on Murashige and Skoog’s basal media supplemented with various combinations of auxins 2,4-dichlorophenoxyacetic acid (2,4-D), -naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole butyric acid (IBA) with kinetin/6-benzyl aminopurine (BA). Callus production was observed in all the media but with varied mass. Highest percentage of callus response was obtained in combination of NAA (1.5 mg L-1) with kinetin (0.5 mg L-1). The friable callus was white in NAA supplemented and brown in 2,4-D and kinetin supplemented media. Three distinct phases viz., lag, exponential or liner and log phase were observed in the growth of callus. Antioxidant activities were analyzed by DPPH, TBARS and metal chelating method. Hyper antioxidant activity was observed in 1-month-old callus produced by NAA in combination with kinetin. The EC50 value of callus extract was 38 ± 3.05 and 54 ± 3.60 by DPPH and TBARS methods, respectively as against 58 ± 2.6 and 64 ±1.2 in in vivo plant material, however, no metal chelating activity was found in both.