Changes in Haemolymph and Egg Protein by the Castration and lmplantation of the Ovary in Bembyx mori

During the pupal development of Bombyx mori, the haemolymph protein content decreases by about one-fourth. Slight change in the concentration was encountered in castrated females throughout pupal-adult development. Ovariectomy applied to females at larval stage does not influence the synthesis of pupal female protein (FP), which normally is found in the female blood of early pupa and then removed quickly from blood during the maturation of the pvaries. Castrated female is characterized by maintaining high FP concentration throghout the pupal life. Ovaries transferred to the hemocoel of males produced eggs but were unable to incorporate FP either into eggs they produced or into the blood of male ovary recipients. These evidences indicate that FP is secreted into the blood by some tissue other than the ovaries, and that it is subsequently transferred from blood to egg yolk. Another deficiency in the protein component of the eggs produced in male-implanted ovary was also demonstrated.