ScholarLens Back to search
2008•Indian Journal of Animal ResearchRequires access

Detection of Brucella DNA in semen using the polymerase chain reaction assay

Amit Kanani, Lata Jain, Tanmay Patel, Dharamsibhai N. Rank, Chaitanya G. Joshi, J. H. Purohit

Open publisher page 2 citations

Abstract

Polymerase chain reaction (PCR) assay was employed for detection of Brucella DNA in semen of cattle and buffalo breeding bulls. A genus specific primer pair which amplified a 223 bp fragment of gene encoding a 31-kDa Brucella abortus antigen was used in assay. Serological test for the diagnosis of Brucella infection was also performed in breeding bulls. The PCR was resulted in more numbers of positive samples as compared to serological test since it detected Brucella DNA in 19 out of 101 semen samples while Brucella antibodies was detected in 10 sera samples of same bulls using ELISA. The PCR may be used as supplementary test for detection of Brucella infection in bulls.

About this research paper

What this paper is about

Polymerase chain reaction (PCR) assay was employed for detection of Brucella DNA in semen of cattle and buffalo breeding bulls. A genus specific primer pair which amplified a 223 bp fragment of gene encoding a 31-kDa Brucella abortus antigen was used in assay. Serological test for the diagnosis of Brucella infection was also performed in breeding bulls. The PCR was resulted in more numbers of positive samples as compared to serological test since it detected Brucella DNA in 19 out of 101 semen samples while Brucella antibodies was detected in 10 sera samples of same bulls using ELISA. The PCR may be used as supplementary test for detection of Brucella infection in bulls.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Polymerase chain reaction (PCR) assay was employed for detection of Brucella DNA in semen of cattle and buffalo breeding bulls. A genus specific primer pair which amplified a 223 bp fragment of gene encoding a 31-kDa Brucella abortus antigen was used in assay. Serological test for the diagnosis of Brucella infection was also performed in breeding bulls. The PCR was resulted in more numbers of positive samples as compared to serological test since it detected Brucella DNA in 19 out of 101 semen samples while Brucella antibodies was detected in 10 sera samples of same bulls using ELISA. The PCR may be used as supplementary test for detection of Brucella infection in bulls.

Key concepts: Semen, Brucella, Polymerase chain reaction, Serology, Primer (cosmetics), Biology, Brucellosis, Virology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Detection of Brucella DNA in semen using the polymerase chain reaction assay — Research Paper | ScholarLens