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2002•Agricultural Sciences in ChinaRequires access

Analysis of Expressed Sequence Tags from Liver Tissue in Swine

Ning Li, Zhihui Zhao, Zhaoliang Liu, Xingbo Zhao, Zhen-Xing Lian, Changxin Wu

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Abstract

In order to study the expression of function gene and its effect on metabolic control and other physiological function in liver, 438 expressed sequence tags (ESTs) were determined, which were from a cDNA library of porcine liver tissue. The results showed that the nucleotide sequences of 186 ESTs have already presented in GenBank database, and 37 ESTs could be found the homology with human and other species, while the others were not identified. 45 full length insertion of the clones randomly isolated from cDNA library were also completely sequenced with different size, and the results showed that 19 of them were function- known genes, 11 had no open reading frame (ORF) at all and 15 had ORF but the function were not elucidated yet.

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What this paper is about

In order to study the expression of function gene and its effect on metabolic control and other physiological function in liver, 438 expressed sequence tags (ESTs) were determined, which were from a cDNA library of porcine liver tissue. The results showed that the nucleotide sequences of 186 ESTs have already presented in GenBank database, and 37 ESTs could be found the homology with human and other species, while the others were not identified. 45 full length insertion of the clones randomly isolated from cDNA library were also completely sequenced with different size, and the results showed that 19 of them were function- known genes, 11 had no open reading frame (ORF) at all and 15 had ORF but the function were not elucidated yet.

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Available abstract

In order to study the expression of function gene and its effect on metabolic control and other physiological function in liver, 438 expressed sequence tags (ESTs) were determined, which were from a cDNA library of porcine liver tissue. The results showed that the nucleotide sequences of 186 ESTs have already presented in GenBank database, and 37 ESTs could be found the homology with human and other species, while the others were not identified. 45 full length insertion of the clones randomly isolated from cDNA library were also completely sequenced with different size, and the results showed that 19 of them were function- known genes, 11 had no open reading frame (ORF) at all and 15 had ORF but the function were not elucidated yet.

Key concepts: GenBank, Open reading frame, Expressed sequence tag, Biology, cDNA library, Complementary DNA, Gene, Homology (biology)

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