ScholarLens Back to search
2000•PubMedRequires access

[Cell cycle arrest and apoptosis induced by arsenic trioxide in human lung cancer cell line].

J. Dong, Yong Wu, Xiaoxv Dong, Liyun Xu, L Liu

Open publisher page 1 citations

Abstract

BACKGROUND: To explore the potential therapeutic effect of arsenic trioxide(As₂O₃ ) on human lung cancer. METHODS: Cell growth curves, cell proliferation, cell cycle and apoptosis of human lung cancer cell line GLC-82 were detected by theMTT method and flowcytometry (FCM) . RESULTS: The data showed that As₂O₃ significantly inhibited proliferation of GLC-82 cells and the inhibiting effects had a dose-and time-dependence. The cell proliferation inhibition rate was 81. 05% when treated with 4. 0 Lmol/L As₂O₃ for 96 hours. The change of DNA content of GLC-82 cells indicated that As₂O₃ was able to block cell cycle progress in G2/M phase, with appearance of sub-G1 peak in a dose-dependent manner. CONCLUSIONS: As₂O₃ can effectively inhibit the proliferation of human lung cancer cell line GLC-82 and the possible mechanisms may be related to G2/M phase arrest and apoptosis induced by As₂O₃.

About this research paper

What this paper is about

BACKGROUND: To explore the potential therapeutic effect of arsenic trioxide(As₂O₃ ) on human lung cancer. METHODS: Cell growth curves, cell proliferation, cell cycle and apoptosis of human lung cancer cell line GLC-82 were detected by theMTT method and flowcytometry (FCM) . RESULTS: The data showed that As₂O₃ significantly inhibited proliferation of GLC-82 cells and the inhibiting effects had a dose-and time-dependence. The cell proliferation inhibition rate was 81. 05% when treated with 4. 0 Lmol/L As₂O₃ for 96 hours. The change of DNA content of GLC-82 cells indicated that As₂O₃ was able to block cell cycle progress in G2/M phase, with appearance of sub-G1 peak in a dose-dependent manner. CONCLUSIONS: As₂O₃ can effectively inhibit the proliferation of human lung cancer cell line GLC-82 and the possible mechanisms may be related to G2/M phase arrest and apoptosis induced by As₂O₃.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

BACKGROUND: To explore the potential therapeutic effect of arsenic trioxide(As₂O₃ ) on human lung cancer. METHODS: Cell growth curves, cell proliferation, cell cycle and apoptosis of human lung cancer cell line GLC-82 were detected by theMTT method and flowcytometry (FCM) . RESULTS: The data showed that As₂O₃ significantly inhibited proliferation of GLC-82 cells and the inhibiting effects had a dose-and time-dependence. The cell proliferation inhibition rate was 81. 05% when treated with 4. 0 Lmol/L As₂O₃ for 96 hours. The change of DNA content of GLC-82 cells indicated that As₂O₃ was able to block cell cycle progress in G2/M phase, with appearance of sub-G1 peak in a dose-dependent manner. CONCLUSIONS: As₂O₃ can effectively inhibit the proliferation of human lung cancer cell line GLC-82 and the possible mechanisms may be related to G2/M phase arrest and apoptosis induced by As₂O₃.

Key concepts: Arsenic trioxide, Apoptosis, Lung cancer, Cell cycle, Cell growth, Cell cycle checkpoint, Cell culture, Cancer research

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
[Cell cycle arrest and apoptosis induced by arsenic trioxide in human lung cancer cell line]. — Research Paper | ScholarLens