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Molecular Cloning of the Restriction Fragments Derived from Double EcoRI/PstI Digestion of the Calf Satellite I DNA and Their Restriction Analysis

Jan Kłysik, Krystyna Furtak, Grażyna Szymczak, Ewa Bartnik, Jacek Skowroński, H Panusz

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Abstract

Calf satellite I DNA digested with EcoRI and PstI gives three fragments 643, 621 and 83 bp long. Two of them, the 643 and 621 bp were cloned using pBR 322 vector and analyzed by means of the MspI and HaeIII restriction enzymes.

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Calf satellite I DNA digested with EcoRI and PstI gives three fragments 643, 621 and 83 bp long. Two of them, the 643 and 621 bp were cloned using pBR 322 vector and analyzed by means of the MspI and HaeIII restriction enzymes.

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Available abstract

Calf satellite I DNA digested with EcoRI and PstI gives three fragments 643, 621 and 83 bp long. Two of them, the 643 and 621 bp were cloned using pBR 322 vector and analyzed by means of the MspI and HaeIII restriction enzymes.

Key concepts: EcoRI, PstI, Restriction enzyme, Restriction digest, Restriction site, Molecular biology, Biology, Restriction map

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Molecular Cloning of the Restriction Fragments Derived from Double EcoRI/PstI Digestion of the Calf Satellite I DNA and Their Restriction Analysis — Research Paper | ScholarLens