2000•PubMedRequires access

Plant regeneration from leaf explants of mulberry: influence of sugar, genotype and 6-benzyladenine.

K. Vijayan, Sajal Chakraborti, Bidhan Roy

Abstract

A protocol for plant regeneration from leaf explants was developed for tropical mulberry varieties. Effect of sugars, 6-benzyladenine and genotype on shoot regeneration was studied. Highest percentage of shoot regeneration (80 +/- 6) was obtained with genotype S799 on medium containing glucose and 8.9 microM 6-benzyladenine. Genotypes Mandalaya and MIHP, having thicker leaves with waxy cuticle, showed poorer regeneration ability than S799 and Sujanpur-5, which have thinner leaves and cuticle. Histological studies revealed that shoots regenerated from sub-epidermal cells.

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Key concepts: Shoot, Explant culture, Biology, Regeneration (biology), Sugar, Botany, Genotype, Cuticle (hair)