2009•Zhongguo Yike Daxue xuebaoRequires access
Effects of pioglitazone on the proliferation and TGF-β1 expression of rat peritoneal mesothelial cells cultured in high glucose concentration.
Lina Yang, Shuo Chen, Jianfei Ma, Fan Yi, Xiuli Zhang, Lining Wang
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Abstract
Objective To observe the effects of pioglitazone on the inhibition of cell proliferation and the expression of transforming growth factor-β1 (TGF-β1)in rat peritoneal mesothelial cells (RPMCs)cultured in high glucose concentration. Methods RPMCs were isolated from rat colic omentum and cultured in high glucose concentration(2.5% glucose). MTT method was processed to observe the effects of pioglitazone (5,10,20μmol/L)on the cell proliferation. ELISA analysis were performed to investigate the change of TGF-β1 concentration in the culture medium. Results High glucose concentration of 2.5% could significantly inhibit cell proliferation as well as induce the expression of TGF-β1 in RPMC(P 0.01),which could be partially reversed by pioglitazone of 5～20 μmol/L(P 0.01). Conclusion Pioglitazone could reverse both the cell proliferation inhibition and fibrosis cytokine TGF-β1 induction by high glucose in RPMC,and repair the cell damage induced by high glucose consequently.