1986•Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)Requires access
mRNA-mediated transfer of genetic information in goldfish eggs
L. C. Niu, Gouxiong Xue, Joy Yang, M. C. Niu
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Abstract
Rabbit globin-mRNA-injected eggs developed red blood cells containing rabbit globin. The mechanism by which the mRNA message was expressed was first suggested by the finding of mRNA transcript (cDNA) in enucleated eggs injected with rabbit globin mRNA. With Southern blotting, this cDNA was hybridized with P/sup 32/-..beta../sub 1/cDNA plasmid (P/sup 32/-pRcB/sub 1/). They found complementary DNA sequence but not in the DNA isolated from uninjected eggs and P/sup 32/pRcB/sub 1/. This result showed the injected-mRNA primed synthesis of rabbit ..beta../sub 1/ cDNA (..beta../sub 1/-globin gene). The fate of the globin cDNA was traced by (1) hybridization of the DNAs from developing stages of the mRNA-injected-and control eggs with P/sup 32/-pRc..beta../sub 1/. They found complementary DNA sequence in the former but not in the latter, thus showing the incorporation of globin cDNA into the DNA of mRNA injected fish, and (2) autoradiograms from sections of the globin H/sup 3/-cDNA (in vitro synthesized)-injected blastula with and without colchicine treatment (5..mu..g/ml). Silver grains were found in areas of nuclei of the control and the colchine treated blastula had them on metaphase chromosomes. Injected albumin mRNA was found to act in accord with the injected-globin-mRNA. The fate of these two mRNA transcripts leads tomore » the proposal that the mRNA transcript in cleaving eggs may be responsible for differential gene activation during early embryogenesis.« less