2009•Journal of Northwest A&F UniversityRequires access
Isolation and identification of two kinds of canker pathogen of Aesculus chinensis.
Yu Zhongdong, Zhen Zhang, Wei He
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Abstract
[Objective] The research studied the isolation and identification of the canker pathogen occurred on Aesculus chinensis Beg.[Method] Traditional Kock's technology and ITS sequence analysis technology were used to ideatify and compare two canker pathogens:NW397 and NW336.[Result] Two kinds of canker pathogen of Aesculus chinensis were identified.Pathogen of immerse canker had over 99% homogeneous with fungi Botryosphaeria dothidea Sacc.Pathogen of dry rot canker had over 95% homogeneous with fungi Phomopsis vaccinii,and so on.[Conclusion] The pathogen of immerse canker (isolate NW397) was identified as Botryosphaeria dothidea Sacc,and the dry rot pathogen (isolate NW336) was identified as Phomopsis fukushii Tanaka et Mill.They were two different pathogen fungi with different disease symptoms.