Sequence analysis of rDNA of anisakid nematodes with zoonotic potential from Taiwan strait.

To analyze the rDNA sequence of the Anisakid nematodes with zoonotic potential from Taiwan straits,Anisakid nematodes were obtained from the gut of marine fish and identified chiefly based on the morphological characteristics.The genomic DNA of nematodes were extracted,and the sequence of rDNA ITS were amplified by PCR using universal primers,then cloned and sequenced bidirectionally.Sequences analysis was conducted by blasting database and software DNAMAN.Results of the blasting database showed that no match could be demonstrated in 3 of 6 rDNA sequences obtained from 6 different nematode species;the new sequences were submitted to the database and the accession numbers in GenBank were obtained.The relationship between adult Contracaecum muraenesoxi and its putative third stage larva was validated by molecular evidences.Sequences relationship based on the inner similarity of the internal transcribed spacer sequences was constructed.It was found that the phylogenetic informative loci were mainly gathered in the second internal transcribed spacer(ITS2) for these anisakid nematodes because of the ITS2 has higher transition ratio than that of the ITS1.It is evident that the sequence in this region could provide valuable information for the molecular distinction of anisakid nematodes with zoonotic potential from Taiwan straits.