2009•Acta Agriculturae ZhejiangensisRequires access
Use of green fluorescent protein gene to label Bacillus subtilis strains.
Weifeng Shen, Baolong Niu, Weng Hongbiao, Yan Liu, Lihua He, Zhiqi Meng
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Abstract
The xylR promoter from B.subtilis 168 chromosomal DNA was ligated with GFP gene from plasmid GFPuv.The expression fragment xylR-GFP was inserted into the shuttle-vector pIM.The recombinant vector pIM-GFP was successfully obtained.The pIM-GFP was transformed into BS523 strain.Transformants were identified by green fluorescence observation with microscope.The results showed that pIM-GFP was successfully integrated into the strain BS523.