1997•Analytical Science and TechnologyRequires access

Determination of Putrescine, Spermidine and Spermine in urine by HPLC

Tae‐Yong Shin, Seok‐Bong Lee, Hyung‐Min Kim

Abstract

A simple and sensitive high performance liquid chromatography for analysis of Putrescine, Spermidine and Spermine in urine is described. The benzoyl chloride derivatives of Putrescine, Spermidine and Spermine are separated on a Bondapak reverse-phase column with methanol/(50/50v/v) as the mobile phase. The results show that the mean level of polyamines in uterine cancer patients urine is much higher than in normal human urine.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Spermidine, Putrescine, Spermine, Urine, Chromatography, Chemistry, High-performance liquid chromatography, Polyamine