2012•The International Journal of Tuberculosis and Lung DiseaseRequires access

Evaluation of nutrient agar for the culture of Mycobacterium tuberculosis using the microcolony detection method [Short communication]

Luqman Satti, S. Abbasi, U. Faiz

Abstract

We evaluated nutrient agar using the microcolony detection method for the recovery of Mycobacterium tuberculosis on 37 acid-fast bacilli (AFB) positive sputum specimens, and compared it with conventional Löwenstein-Jensen (LJ) medium. Nutrient agar detected 35 isolates compared to 34 on LJ medium. The mean time to detection of mycobacteria on nutrient agar and LJ medium was respectively 9.6 and 21.4 days. The contamination rate on nutrient agar and LJ medium was respectively 5.4% and 2.7%. Nutrient agar detects M. tuberculosis more rapidly than LJ medium, and could be an economical, rapid culture method in resource-poor settings, provided our findings are confirmed by further studies.

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Key concepts: Nutrient agar, Agar, Sputum, Microbiology, Mycobacterium tuberculosis, Bacilli, Nutrient, Tuberculosis