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2011•Journal of Food Hygiene and SafetyRequires access

Analysis and Survey for Contamination of Deoxynivalenol and Zearalenone in Feed by High Performance Liquid Chromatography

Dong-Ho Kim, Kyu-Il Choi, Kyung-Suk Hong, Hyun Jung Kim, Yeong-Jin Song, Seung-Hun Gang, Han-Sub Jang, Hyun‐Jung Cho, Gye-Su Han

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Abstract

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high perfor- mance liquid chromatography/fluorescence with diode array detector (HPLC /FLD with DAD). The average recover- ies of DON were 88.76 and 95.40% at the levels of 200 and 1,000 µg/kg and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 µg/kg, respectively. The limit of detection (LOD) were 6.0 and 3.0 µg/kg for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 µg/kg in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 µg/kg for them, individually.

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What this paper is about

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high perfor- mance liquid chromatography/fluorescence with diode array detector (HPLC /FLD with DAD). The average recover- ies of DON were 88.76 and 95.40% at the levels of 200 and 1,000 µg/kg and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 µg/kg, respectively. The limit of detection (LOD) were 6.0 and 3.0 µg/kg for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 µg/kg in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 µg/kg for them, individually.

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Available abstract

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high perfor- mance liquid chromatography/fluorescence with diode array detector (HPLC /FLD with DAD). The average recover- ies of DON were 88.76 and 95.40% at the levels of 200 and 1,000 µg/kg and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 µg/kg, respectively. The limit of detection (LOD) were 6.0 and 3.0 µg/kg for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 µg/kg in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 µg/kg for them, individually.

Key concepts: Zearalenone, Mycotoxin, Contamination, High-performance liquid chromatography, Chromatography, Detection limit, Vomitoxin, Chemistry

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