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Hyoscyamus-Nicotiana Fusion Hybrids Selected via Auxotroph Complementation and Verified by Species-Specific DNA Hybridisation

Jingying Jia, Ingo Potrykus, G. Lázár, Michael W. Saul

Open publisher page 9 citations

Abstract

With efficient fusion methods available (1, 2), the limiting step in somatic hybridisation is selection. Complementation of metabolic deficiencies of both fusion partners would allow for a continuous selection pressure for fusion hybrids. A series of auxotrophs isolated from haploid mesophyll protoplasts of Hyoscyamus muticus in this institute (3, 4) provided the material to test whether auxotroph complementation would provide a reliable method for somatic hybrid selection in higher paints.

About this research paper

What this paper is about

With efficient fusion methods available (1, 2), the limiting step in somatic hybridisation is selection. Complementation of metabolic deficiencies of both fusion partners would allow for a continuous selection pressure for fusion hybrids. A series of auxotrophs isolated from haploid mesophyll protoplasts of Hyoscyamus muticus in this institute (3, 4) provided the material to test whether auxotroph complementation would provide a reliable method for somatic hybrid selection in higher paints.

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OpenAlex reports 9 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

With efficient fusion methods available (1, 2), the limiting step in somatic hybridisation is selection. Complementation of metabolic deficiencies of both fusion partners would allow for a continuous selection pressure for fusion hybrids. A series of auxotrophs isolated from haploid mesophyll protoplasts of Hyoscyamus muticus in this institute (3, 4) provided the material to test whether auxotroph complementation would provide a reliable method for somatic hybrid selection in higher paints.

Key concepts: Auxotrophy, Complementation, Protoplast, Somatic fusion, Biology, Hybrid, Cell fusion, Selection (genetic algorithm)

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