Determination of Thiamine in Urine and Feces of the Rats by the Fluorometric Method
Hyun-Ki Lee, Sook‐Hee Rhee
Abstract
Hyun-Ki Lee, Sook‐Hee Rhee
Abstract
Two 3-month old Wistar Strain male rats (weights was 270g and 340g respectively) were used as samples in order to determine the daily excretion of thiamine contained in urine and feces by the thiochrome reaction. The results were as follows. 1. Daily excretion of urine was 5,2 cc per 100 grams of body weight and that of thiamine was per 100 grams of plasma. 2. Daily excretion of feces was 2.4 grams per 100 grams of body weight and that of thiamine was per 100 grams of plasma. 3. Daily excretion of thiamine contained in urine is about twice the amount of thiamine excretion contained in feces. Thus, it can be concluded that more of the excretion of thiamine was mainly excreted through urine.
A significance statement is not available in the OpenAlex record.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
Two 3-month old Wistar Strain male rats (weights was 270g and 340g respectively) were used as samples in order to determine the daily excretion of thiamine contained in urine and feces by the thiochrome reaction. The results were as follows. 1. Daily excretion of urine was 5,2 cc per 100 grams of body weight and that of thiamine was per 100 grams of plasma. 2. Daily excretion of feces was 2.4 grams per 100 grams of body weight and that of thiamine was per 100 grams of plasma. 3. Daily excretion of thiamine contained in urine is about twice the amount of thiamine excretion contained in feces. Thus, it can be concluded that more of the excretion of thiamine was mainly excreted through urine.
Key concepts: Thiamine, Excretion, Urine, Feces, Chemistry, Biology, Biochemistry, Microbiology