Proteome Analysis of Hirschsprung Disease by Two-dimensional Electrophoresis

Objective To study the protein expression profiles in stegnotic and normal segment of Hirschsprung disease(HD)and find the differentially expressed proteins. Methods Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis (2-DE)was used to isolate the proteins from stegnotic and normal segment of HD. After the samples were treated with silver staining,ImageMaster 2D Platinum analysis software was used to analyze the differentially expressed proteins. Results Repeatable 2-DE profiles were obtained. The mean matching rates of the stegnotic and normal mucosa were 78.1% and 86.7 %,respectively. Totally,103 spots of differentially expressed pro-teins were screened out between the stegnotic and the normal segment of HD. Conclusion Good reproducibility and resolution could be obtained in the tissues of HD by applying immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis.Screened differentially expressed proteins may provide the candidates of the markers for early detection of HD.