核因子－κB p65在小鼠耳蜗中的表达

Objective Nuclear factor-κB (NF-κB) is one of the best-characterized transcription factors playing an important role in many cell responses to a large variety of stimuli. The aim of this study is to show the capability of NE-κB expression in cochlea. Methods Kanamycin (KA) was subcutaneuously injected twice daily for 3 and 7 days with an eight hour interval between two injections. Lipopolysaccharides (LPS) was injected into the tympanic cavity of mice. Equal saline was injected for 7 day as control. Cryosection of all mice cochlear was examined immmunohistochemichally with rabbit polyconal NF-κB p65. Cololization of NF-κB p65 immunoreactivity of mouse cochlear was identified and appeared as the brown reaction products characteristic of DAB immunohistochemistry. Results Immnoreactivity NF-κB p65 in mouse cochlea was colocalized in the organ of Corti, spiral limbus, tectorial membrane, the stria vascularis, spiral ligament, spiral ganglion and nerve fibers. The immunoreaction could be observed in all turns throughout the cochlea. Stronger staining was visible in spiral ligament, tectorial membrane, spiral prominence, spiral ganglion and nerve fibers, and the organ of Corti. The immunoreaction in the stria vascularis was weaker than that of above mentioned structures. The immunoreaction in the organ of Corti was observed in inner hair cells (IHC) and outer hair cells (OHC), inner and outer pillar cells, Deiter's cells, and Boettcher's cells. The immunoreaction was weaker in innersulcus cells, Hensen's cells and Claudius' cells. The stronger immunoreaction was observed in nucleus of spiral ganglion cells. The nucleus of IHC and OHC remains unstained. No colocalization of NF-κB p65 immunoreactivity appeared in mouse cochlea when the primary antibody was omitted. Significant differences between saline and LPS/KA-treated cochlea concerning NE-κB p65 expression could be observed. There was no significant difference in NF-κB p65 expression of LPS/KA-treated cochlea for 3 and 7 days. Conclusion The density positive expression of NF-κB p65 in the mouse cochlea can be induced by LPS and KA injection.