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2004•Journal of Animal and Feed SciencesOpen access

Gene expression in bovine mammary somatic cells isolated from milk

Alyssa Hayashi, S. A. McCoard, Nicole C. Roy, Matthew P. G. Barnett, Duncan S. MacKenzie, Warren C. McNabb

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Abstract

Traditionally, in order to examine the expression of genes involved in mammary gland metabolism, RNA was prepared from mammary biopsies or from tissue samples taken at slaughter.The objective of this study was to validate the use of milk somatic cells as a model to study gene expression in the lactating bovine mammary gland.The expression pattern of milk protein and epithelial cell marker genes was similar between somatic cells and mammary tissue indicating that cells exfoliated into milk provide a viable non-invasive tissue source for gene profi ling in the bovine mammary gland.

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Traditionally, in order to examine the expression of genes involved in mammary gland metabolism, RNA was prepared from mammary biopsies or from tissue samples taken at slaughter.The objective of this study was to validate the use of milk somatic cells as a model to study gene expression in the lactating bovine mammary gland.The expression pattern of milk protein and epithelial cell marker genes was similar between somatic cells and mammary tissue indicating that cells exfoliated into milk provide a viable non-invasive tissue source for gene profi ling in the bovine mammary gland.

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Available abstract

Traditionally, in order to examine the expression of genes involved in mammary gland metabolism, RNA was prepared from mammary biopsies or from tissue samples taken at slaughter.The objective of this study was to validate the use of milk somatic cells as a model to study gene expression in the lactating bovine mammary gland.The expression pattern of milk protein and epithelial cell marker genes was similar between somatic cells and mammary tissue indicating that cells exfoliated into milk provide a viable non-invasive tissue source for gene profi ling in the bovine mammary gland.

Key concepts: Udder, Mammary gland, Somatic cell, Mammary tissue, Gene, Gene expression, Biology, Andrology

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