2001•PubMedRequires access
[Restriction analysis of plasmid of the strain Streptomyces sp.27].
V V Luk'ianchuk, Л. В. Поліщук, G M Stryzhkova, B P Matseliukh
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Abstract
Plasmid pSS27 was found in streptomycete strain isolated from the soil sample in the Kyiv region. The paper deals with the data of restriction analysis of streptomycete plasmid pSS27 and its molecular weight. Plasmid pSS27 has unique restriction sites for endonucleases BglII and EcoRI, there are also the restriction sites for SalGI (3 sites), PstI (4 sites), BglI (5 sites), Bsp120I (7 sites) and SacII (11 sites). The molecular weight of plasmid DNA pSS27, being equal to 13.7 +/- 0.2 kb has been determined as a result of its restriction analysis.