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The comparison between four potato cultivars multiple axillary bud micropropagation system efficiency.

O. Danci, M. Danci

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Abstract

The growth in vitro of several potato cultivars (Amelia, Christian, Nicoleta and Roclas) on media containing plant growth regulators has been studied with a view to accelerating micropropagation of slow-growing cultivars. Whilst 0,5 mg/l GA3+0.1 mg/l IAA substantially increased the height of plantlets of most cultivars, the combination of 0,1 mg/l GA3+0,1 mg/l IAA+ 1,5 mg/l BAP was more effective in increasing the number of shoots which could subsequently be cultured. Using PM medium, a specific medium designed for potato, slow-growing or recalcitrant cultivars, such as Amelia, could be multiplied more rapidly than on basic Murashige & Skoog medium. Cultivars studied showed wide variation in their response to the plant regulators, best results being obtained for the cultivar Nicoleta.

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What this paper is about

The growth in vitro of several potato cultivars (Amelia, Christian, Nicoleta and Roclas) on media containing plant growth regulators has been studied with a view to accelerating micropropagation of slow-growing cultivars. Whilst 0,5 mg/l GA3+0.1 mg/l IAA substantially increased the height of plantlets of most cultivars, the combination of 0,1 mg/l GA3+0,1 mg/l IAA+ 1,5 mg/l BAP was more effective in increasing the number of shoots which could subsequently be cultured. Using PM medium, a specific medium designed for potato, slow-growing or recalcitrant cultivars, such as Amelia, could be multiplied more rapidly than on basic Murashige & Skoog medium. Cultivars studied showed wide variation in their response to the plant regulators, best results being obtained for the cultivar Nicoleta.

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Available abstract

The growth in vitro of several potato cultivars (Amelia, Christian, Nicoleta and Roclas) on media containing plant growth regulators has been studied with a view to accelerating micropropagation of slow-growing cultivars. Whilst 0,5 mg/l GA3+0.1 mg/l IAA substantially increased the height of plantlets of most cultivars, the combination of 0,1 mg/l GA3+0,1 mg/l IAA+ 1,5 mg/l BAP was more effective in increasing the number of shoots which could subsequently be cultured. Using PM medium, a specific medium designed for potato, slow-growing or recalcitrant cultivars, such as Amelia, could be multiplied more rapidly than on basic Murashige & Skoog medium. Cultivars studied showed wide variation in their response to the plant regulators, best results being obtained for the cultivar Nicoleta.

Key concepts: Cultivar, Micropropagation, Axillary bud, Shoot, Biology, Plant growth, Horticulture, Botany

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