Laboratory Evaluation of Entomopathogenic Nematodes, Steinernema carpocapsae and Heterorhabditis bacteriophora against Some Forest Insect Pests

The pathogenicity of entomopathogenic nematodes Sfeinernema earpocapsae and Heferorhabditis baeferiophora was evaluated against forest insect pests, alder leaf beetle Agelastiea eoerulea, pellucid zygaenid Pryeria siniea, and box-tree pyralid Glyphodes perspeetalis. Alder leaf beetle larvae were exposed to S. earpocapsae at concentration of 0, 25, 50, and 100 nematodes and to H. baeferiophora at concentration of 0, 10, 20, and 40 nematodes per larva on alder leaves. Mortalities of 1st instar lavae were 85.4 4.1-100%, 2nd instar larvae 80.0 5.8-100%, and 3rd instar larvae 65.0 10.8-100% in S. earpocapsae and those of 1st instar larvae were 82.5 6.9-100%, 2nd instar larvae 77.5 4.7-100%, and 3rd instar larvae 55.0 13.5-100% in H. baeferiophora treatment. When pellucid zygaenid larvae were exposed to S. earpocapsae at concentration of 0, 5, 10, 20, 40, and 80 nematodes and to H. baeteriophora at concentration of 0, 2, 5, 10,20, and 40 nematodes per larva, mortalities were 98.9 1.1-100% in S. earpocapsae and 26.7 5.1-74.5 6.2% in H. baeferiophora. The mortalities of box-tree pyralid larvae were 97.8 1.5-100% in S. earpocapsae treated with concentration of 0,20,40, and 80 nematodes per larva and those were 92.0 6.2-98.9 1.1 % in H. baeferiophora treated with con'||'&'||'not;centration of 0, 10, 20, and 40 nematodes per larva.