2000•Glasnik Sumarskog fakultetaRequires access

Stem segment in vitro culture of chrysanthemum as a method for micropropagation

Ljiljana Radojević, Slađana B. Jevremović, Biljana Lazić

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Abstract

The effect of several nutritional media on in vitro shoot multiplication of 13 chrysanthemum cultivars grown in our country was investigated. Medium supplemented with a-naphtalenacetic acid and 6-benzylaminopurine was the most efficient for shoot multiplication. The highest shoot multiplication index was achieved for cultivar 'Tigerrag'. Successful rooting of shoots, depending on cultivars, was obtained on a hormone free medium. Acclimatization of 'in vitro' plants was 55-84%. After growing in the field under suitable photoperiod regime, all cultivars flowered.

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What this paper is about

The effect of several nutritional media on in vitro shoot multiplication of 13 chrysanthemum cultivars grown in our country was investigated. Medium supplemented with a-naphtalenacetic acid and 6-benzylaminopurine was the most efficient for shoot multiplication. The highest shoot multiplication index was achieved for cultivar 'Tigerrag'. Successful rooting of shoots, depending on cultivars, was obtained on a hormone free medium. Acclimatization of 'in vitro' plants was 55-84%. After growing in the field under suitable photoperiod regime, all cultivars flowered.

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Available abstract

The effect of several nutritional media on in vitro shoot multiplication of 13 chrysanthemum cultivars grown in our country was investigated. Medium supplemented with a-naphtalenacetic acid and 6-benzylaminopurine was the most efficient for shoot multiplication. The highest shoot multiplication index was achieved for cultivar 'Tigerrag'. Successful rooting of shoots, depending on cultivars, was obtained on a hormone free medium. Acclimatization of 'in vitro' plants was 55-84%. After growing in the field under suitable photoperiod regime, all cultivars flowered.

Key concepts: Shoot, Cultivar, Micropropagation, Acclimatization, Biology, Botany, Murashige and Skoog medium, Explant culture

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