Stem segment in vitro culture of chrysanthemum as a method for micropropagation
Ljiljana Radojević, Slađana B. Jevremović, Biljana Lazić
Abstract
Ljiljana Radojević, Slađana B. Jevremović, Biljana Lazić
Abstract
The effect of several nutritional media on in vitro shoot multiplication of 13 chrysanthemum cultivars grown in our country was investigated. Medium supplemented with a-naphtalenacetic acid and 6-benzylaminopurine was the most efficient for shoot multiplication. The highest shoot multiplication index was achieved for cultivar 'Tigerrag'. Successful rooting of shoots, depending on cultivars, was obtained on a hormone free medium. Acclimatization of 'in vitro' plants was 55-84%. After growing in the field under suitable photoperiod regime, all cultivars flowered.
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The effect of several nutritional media on in vitro shoot multiplication of 13 chrysanthemum cultivars grown in our country was investigated. Medium supplemented with a-naphtalenacetic acid and 6-benzylaminopurine was the most efficient for shoot multiplication. The highest shoot multiplication index was achieved for cultivar 'Tigerrag'. Successful rooting of shoots, depending on cultivars, was obtained on a hormone free medium. Acclimatization of 'in vitro' plants was 55-84%. After growing in the field under suitable photoperiod regime, all cultivars flowered.
Key concepts: Shoot, Cultivar, Micropropagation, Acclimatization, Biology, Botany, Murashige and Skoog medium, Explant culture