2015•PubMedRequires access

Accuracy of a vancomycin brain heart infusion screening plate for the screening of Staphylococcus aureus isolates with increased vancomycin minimum inhibitory concentrations.

Alessandra Navarini, Marinês Dalla Valle Martino, Suzete Matiko Sasagawa, Irineu F D Massaia, Marcelo Jenné Mimiça

Abstract

Vancomycin susceptibility was determined in 125 S. aureus isolates by disk diffusion, microdilution, Etest and vancomycin brain heart infusion (BHI) plate. A 2.0 mg/L vancomycin BHI was highly sensitive (100% and 91% compared to Etest and microdilution) for detecting a MIC≥2 mg/L, and could be used as a simple and affordable screening test.

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Key concepts: Etest, Vancomycin, Broth microdilution, Brain heart infusion, Minimum inhibitory concentration, Staphylococcus aureus, Microbiology, Medicine