1992•Medical Entomology and ZoologyRequires access
Laser light scattering in biochemistry
Stephen E. Harding, D. B. Sattelle, Victor A. Bloomfield
Open publisher page 165 citations
Abstract
Part 1 Techniques: static and dynamic light scattering approaches to structure determination of biopolymers the use of low angle laser light scattering with gel permeation chromatography for the molecular weight determination of biomolecules combined differential light scattering with various liquid chromatography separation techniques new tools of biochemists - combined laser doppler microelectrophoresis and photon correlation spectroscopy an on-line dynamic scattering instrument for macromolecular characterization an overview of current methods of analyzing QLS data low-bias macroscopic analysis of polydispersity mesoparticle diffusion of biopolymer and polymer solutions the diffusion of BSA molecules on poly(N-isopropyl acrylamide) gels - a forced Rayleigh scattering study developments in electrophoretic laser light scattering and some biochemical applications an investigation of rigid rod-like particles in dilute solution static and dynamic light scattering by semi-dilute rod-like polyelectrolytes quasi-elastic light scattering from an evanescent wave to probe particle/wall interactions. Part 2 Macromolecules: compactness of protein in molecules in native and denatured states as revealed by laser light scattering and X-ray scattering dynamic light scattering studies of concentrated Casein Micelle suspensions aggregation of proteins hydrodynamic behaviour of myosin filaments studied by laser light scattering quasielastic and total intensity light scattering studies of mucin glycoproteins and cartilage proteoglycans use of on-line laser light scattering coupled to chromatographic separations for the determination of molecular weight, branching, size and shape distributions of polysaccharides chain rigidity of polyuronates - static light scattering of aqueous solutions of hyaluronate and alginate laser light scattering studies of polysaccharide gels light scattering studies of DNA condensation dynamics and structures of DNA DNAs as models of rigid and semi-rigid rod-like macromolecules. Part 3 Macromolecular assemblies: total intensity and quasi-elastic light scattering applications in microbiology.