Development of Ecotoxicological Standard Methods using Early Life Stage of Marine Rotifer Brachionus plicatilis and Benthic Copepod Tigriopus japonicus

Marine ecotoxicological standard method was applicated using marine rotifer and benthic copepod as primary consumer of marine ecosystem. Marine rotifer, Brachionus plicatilis and benthic copepod, Tigriopus japonicus were designated as standard species with the endpoints of 24hr neonate mortality (24hr ) and 48hr population growth (48hr ) for rotifer, and 48hr nauplius mortality (48hr ) for benthic copepod. Tests method was referred to those of ASTM (American Society for Testing and Materials) with the replacement of species which are widely distributed in Korean waters. The two species showed a wide tolerance on salinity () and can be easily cultured in small space even they were not as sensitive as in the mortality using the nauplius of marine invertebrates. However, these species revealed the significant concentrationresponse relationship tested with ocean disposal wastes, and reproducibility using cadmium as standard reference material between laboratories. Accordingly, these two species have good potential as species for marine ecotoxicological species. Also, we strongly recommend that at least one of these species be included in the species of battery test in marine bioassay.